Description_x000D_ General description_x000D_ CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays._x000D_ Features and Benefits_x000D_ • Efficient: Up to 50% more efficient than freeze thaw, sonication and other products_x000D_ • Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays_x000D_ • Convenient: Ready-to-use reagent requires no scraping from culture plates_x000D_ • Fast: Rapid cell lysis at room temperature._x000D_ Quantity_x000D_ CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC)._x000D_ Analysis Note_x000D_ Use 125 μl CelLytic M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate._x000D_ Legal Information_x000D_ CelLytic is a trademark of Sigma-Aldrich Co. LLC