Description_x000D_ General description_x000D_ Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of Fungi and exoskeketal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects, fungi, yeast, and algae, and in the internal structures of other vertebrates) . Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source.Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source._x000D_ The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:_x000D_ • The first reaction, chitodextrinase-chitinase, is a poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase, which removes chitobiose units from chitin._x000D_ • The second activity is N-acetyl-glucosaminidasechitobiase, which cleaves the disaccharide to its monomer subunits, N-acetyl-D-glucosamine._x000D_ Application_x000D_ Agriculture fields: control pathogens._x000D_ Human health care: Asthma._x000D_ Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine,_x000D_ Preparation of single-cell protein_x000D_ Isolation of protoplasts from fungi and yeast_x000D_ Control of pathogenic fungi_x000D_ Treatment of chitinous waste, mosquito control and morphogenesis_x000D_ Packaging_x000D_ 5, 25, 50 units in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Chitinase is an extracellular enzyme complex that degrades chitin and has a molecular mass of approximately 30 kDa. Chitin is degraded to N-acetyl-D-glucosamine in 2 enzymatic reactions. Firstly, chitobiose units are removed from chitin by chitodextrinase-chitinase. The second reaction involves N-acetyl-glucosaminidase-chitobiase, which cleaves the disaccharide to its monomer subunits (that comprise of N-acetyl-D-glucosamine). The optimum reaction temperature is 37 °C._x000D_ Features and Benefits_x000D_ Chitinase is an extracellular complex of enzymes that degrade chitin. It is a lytic enzyme suitable for fungal cell walls lysis._x000D_ Unit Definition_x000D_ One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 °C in a 2 hour assay._x000D_ One new 1 hour unit = approx. 50 old 48 hour units._x000D_ Other Notes_x000D_ View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer