Description_x000D_ General description_x000D_ Reverse Cholesterol Transport (RCT) is the mechanism by which the peripheral lipid burden is reduced by transport to liver and its fecal excretion. The first important step in RCT is the cholesterol efflux from macrophage-derived foam cells present in atherosclerotic plaques. The cholesterol efflux is critical for the maintenance of net cholesterol balance in arterial walls and in the reduction of proinflammatory responses triggered by lipid-laden macrophages._x000D_ Application_x000D_ Cholesterol Efflux Assay Kit has been used in cholesterol efflux assay._x000D_ Suitability_x000D_ Suitable for the determination of cholesterol efflux in cells._x000D_ Principle_x000D_ The Cholesterol Efflux Assay Kit is a high-throughput screening assay that measures cholesterol efflux in cells using fluorescent-labeled cholesterol (λex = 482/ λem = 515 nm). It is suitable for serum, isolated, or recombinant lipoprotein samples. It may also be used to screen small molecules for their effect on cholesterol efflux and therefore, may be a valuable tool in drug discovery programs.