Description_x000D_ General description_x000D_ The CompoZr Targeted Integration Kit – AAVS1 is designed to rapidly integrate a user-specified gene of interest (GOI) into the adeno-associated virus integration site 1 (AAVS1) on human chromosome 19. The Zinc Finger Nuclease (ZFN) in this kit targets the AAVS1 site with high specificity, creating a double strand break. The cell then uses the pZDonor plasmid, with homology arms to the AAVS1 genomic region, in homology-directed repair to replace the natural AAVS1 locus. The pZDonor plasmid can be engineered to contain your GOI and will direct targeted integration of your GOI into the AAVS1 locus._x000D_ Features and Benefits_x000D_ •Effective integration efficiency without antibiotic selection_x000D_ The ZFNs engineered to target AAVS1 are a well-validated pair that cut the DNA with high efficiency. ZFN-aided targeted integration can achieve 20% efficiency in some cell types without antibiotic selection, representing an improvement of three to four orders of magnitude in efficiency over normal homologous recombination events1._x000D_ •Rapid biallelic insertions through a single transfection_x000D_ ZFN mediated homology-directed repair leads to gene addition within 48 hours of transfection of ZFN mRNA/plasmid donor, with both monoallelic and biallelic insertions1._x000D_ •Controlled transgene effects through use of single genomic locus_x000D_ In the past, most transgenic cell lines have been constructed via random integration of a plasmid. This results in a collection of clones with greatly varied expression levels and expression stability. Targeted integration into the AAVS1 locus will provide a common integration site with stable, uniform expression levels1._x000D_ • Eliminates need to engineer cell lines with transgene landing pads_x000D_ Transgene integration into the genome has previously been achieved via recombination through recombinases such as Cre and FLP. This requires prior insertion of a transgene landing pad into the genome through random integration followed by transgene addition, making it a lengthy two step process. Targeted integration into AAVS1 eliminates the first step, making transgene expression a simple 1-step process1._x000D_ Legal Information_x000D_ All Zinc Finger Nucleases are sold under license from Sangamo Biosciences. For a copy of the Label License provided with purchase of CompoZr ZFNs, please visit the ZFN Label License page._x000D_ CompoZr is a registered trademark of Sigma-Aldrich Co. LLC