Description_x000D_ Application_x000D_ Creatine Phosphokinase from bovine heart has been used:_x000D_ • in the reaction mix for the import of in vitro synthesized wild-type FLAG/MYC-tagged LYR Motif Containing 7 (LYRM7-F/M) or the LFK tripeptide replaced with alanine (LFK-AAA) mutant into isolated mitochondria_x000D_ • in autoubiquitination assay_x000D_ • in the preparation of premix buffer for adenylyl cyclase activity_x000D_ • in the preparation of E-mix for in vitro nuclear assembly and isolation_x000D_ Creatine phosphokinase from bovine heart has been used to investigate whether endothelial cell growth is stimulated by ischemic hearts. Creatine phosphokinase from bovine heart has also been used to evaluate the effect of high but nontoxic dietary intake of copper and selenium on metabolism in calves._x000D_ The product has been used for tATPase assay of myofibrillar protein isolated from rabbit. This assay evaluated the kinetic influence of bound creatine kinase (CK) on Ca2+-activated myosin ATPase. The product has also been used for the enzymatic hydrolysis of protein samples during tryptophan estimation by pyrolysis gas chromatography._x000D_ Packaging_x000D_ 500, 1000, 3500, 17500 units in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP. The molecular mass of the protein is found to be approximately 80,000 Da. It is made up of 2 subunits, each having a molecular weight of 40,000 ± 2000. The lighter subunit is present in larger amounts._x000D_ Caution_x000D_ The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution._x000D_ Unit Definition_x000D_ One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C._x000D_ Analysis Note_x000D_ Protein determined by biuret.