Description General description Biologically active, cell-permeable, non-physiological ceramide analog. Induces a dramatic arrest in the G0/G1 phase of the cell cycle. Activates MAP kinase. Activates protein phosphatase 2A (PP2A) at 10 nM. Inhibits diacylglycerol accumulation and phospholipase D (PLD) activation in fibroblasts. Induces apoptosis in MOLT-4 leukemia cells. Biologically active, cell-permeable, nonphysiological ceramide analog. Stimulates cytosolic serine/threonine protein phosphatase in T9 cells and induces phosphorylation on Thr-669 in A-431 cells by stimulation of ceramide-activated protein kinase. Induces a dramatic arrest in the Go/G1 phase of the cell cycle. Activates MAP kinase. Activates protein phosphatase 2A (PP2A) at 10 nM. Inhibits diacylglycerol accumulation and phospholipase D activation in fibroblasts. Induces apoptosis in Molt-4 leukemia cells. Packaging 5 mg in Plastic ampoule Biochem/physiol Actions Cell permeable: yes Effective concentration: 10 nM in activating protein phosphatase 2A (PP2A) Primary Target MAP kinase, protein phosphatase 2A (PP2A) Product does not compete with ATP. Reversible: no Warning Toxicity: Irritant (B) Reconstitution Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C. Other Notes Jayadev, S., et al. 1995. J. Biol. Chem. 270, 2047. Venable, M.E., et al. 1994. J. Biol. Chem. 269, 26040. Dobrowsky, R.T., et al. 1993. J. Biol. Chem. 268, 15523. Raines, M.A., et al. 1993. J. Biol. Chem.268, 14572. Dobrowsky, R.T., and Hannun, Y.A. 1992. J. Biol. Chem.267, 5048.