Description Preparation Note Toluene / Dichloroacetic acid 97/3 (v/v) Other Notes Deblock solution containing dichloroacetic acid (DCA) in toluene removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction. Legal Information OligoPilot is a registered trademark of Cytiva ÄKTA is a registered trademark of Cytiva