Description Packaging 4×4 L in glass bottle 6×450 mL in glass bottle Preparation Note Dicyanoimidazole / Acetonitrile 3/100 (w/v) Other Notes Activator solution, containing 4,5-dicyanoimidazole (DCI) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.