Description_x000D_ Application_x000D_ Deoxyribonuclease II from bovine spleen has been used as a component of the dissociation medium in the cell preparation of neuro-intermediate lobe of the pituitary gland._x000D_ Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes._x000D_ Packaging_x000D_ 30000, 150000, 300000 units in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. In vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da._x000D_ Unit Definition_x000D_ One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM