Description_x000D_ General description_x000D_ Phage DNA is isolated from infected E. coli, passed through a series of enzymatic steps before final phenol-chloroform extraction. This methylated lambda DNA is partially cleaved by Bcl I, Cla I, Mbo I, Mbo II, Taq I and Xba I._x000D_ Specificity_x000D_ Unique restriction sites: Apa I,Nae I, Nar I, Nhe I, PaeR7 I, SnaB I, Xba I and Xho I. Methylated lambda is partially cleaved by Bcl I, Cla I, Mbo I, Mbo II, Taq I and Xba I._x000D_ Application_x000D_ Lambda Phage DNA, Methylated from Escherichia coli host strain W3110 has been used in DNA combing to determine the conversion factor._x000D_ Lambda Phage DNA, Methylated from Escherichia coli host strain W3110 is suitable for use as a substrate for restriction enzymes. It was used for immunoprecipitation in ChIP assays._x000D_ The lambda phage has an icosahedral head and a long tail terminating in a single fiber. At both ends of the 5′ termini are complementary 12-nucleotide single strand sequences that contribute to the cohesive ends (cos region) of the DNA. The tail of the phage latches on the host outer membrane receptor and injects phage DNA into the cell. The phage converts the E. coli to a lysogenic state in which the phage functions are repressed and the phage genome may remain dormant (prophage) for a long time. This property is seen in bacteriophages that carry CII and CIII genes that are responsible for CI expression. Bacteriophages with CI mutation in the CI gene are able to maintain a lysogenic state at defined temperatures._x000D_ Infecting E. coli strain W3110 with lambda C1857 strain creates E. coli lysogen cultures. The phage is released from E. coli cell pellets by lysing with a high salt buffer, pH 8.0. The crude mixture is passed through a series of enzymatic steps, multiple cesium gradients, and phage DNA is dialyzed against 1 mM Tris-HCl, pH 8.0, and 1 mM magnesium chloride. The DNA is finally extracted by phenol-chloroform solution._x000D_ Packaging_x000D_ 1 unit in glass bottle_x000D_ 5, 10 units in glass bottle_x000D_ Unit Definition_x000D_ One O.D. (A260) is approximately 50 μg of DNA_x000D_ Reconstitution_x000D_ Add the appropriate amount of 1x Tris-EDTA buffer, pH 8.0 (Product No. T9285, diluted to 1x, or comparable), to the vial._x000D_ Incubate the DNA sample vial at 65 °C for 4 hrs with frequent mixing._x000D_ After reconstitution, the product should be stored at -20 °C.