Description_x000D_ General description_x000D_ Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen._x000D_ Application_x000D_ Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms._x000D_ Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases._x000D_ Packaging_x000D_ 5, 25, 50, 100 mL in glass bottle_x000D_ Biochem/physiol Actions_x000D_ DEPC is a potent nuclease inhibitor.0.1% solution of DEPC is used to inactive RNase._x000D_ It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose and time dependent manner and increases the Ca2+ permeability of SR vesicles._x000D_ Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.