Description_x000D_ General description_x000D_ Features and Benefits:_x000D_ • Suitable for Molecular Applications_x000D_ • Nuclease-Free DMSO_x000D_ • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free_x000D_ • Molecular Biology Grade DMSO_x000D_ Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals._x000D_ This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases._x000D_ Application_x000D_ DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in_x000D_ • polymerase chain reaction (PCR)_x000D_ • amplification of cDNA libraries_x000D_ • DNA sequencing_x000D_ • column-loading buffers for poly(A)+ RNA selection_x000D_ • buffers for the transformation of competent E. coli_x000D_ • transfection protocols._x000D_ Dimethyl sulfoxide has been used-_x000D_ • as an oligonucleotide solvent in array spotting[1]_x000D_ • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)_x000D_ • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay_x000D_ • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment_x000D_ • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent._x000D_ To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended._x000D_ Packaging_x000D_ 1 L in glass bottle_x000D_ 50, 100, 250, 500 mL in glass bottle_x000D_ Features and Benefits_x000D_ This product is free from DNases, RNases, phosphatases and proteases._x000D_ Caution_x000D_ Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.