Description Application DL-Buthionine-sulfoximine is suitable for use to: • examine whether the inhibition of glutathione by BSO enhances the apoptotic effect of estrogen on antihormone-resistant human breast cancer cells • investigate the effect of BSO on development of bovine embryos • inhibit GSH in several studies • investigate the effect of GSH synthesis on oocyte maturation It may be used to inhibit GSH and evaluate the hepatotoxicity and nephrotoxicity of natural food colorants in the absence of GSH Biochem/physiol Actions DL-Buthionine-sulfoximine inhibits the biosynthesis of Glutathione (GSH) in liver and other peripheral organs. It does not have any effect on GSH in the CNS. It augments the antiproliferative action of reactive oxygen species (e.g., hydrogen peroxide), and agents that indirectly cause accumulation of reactive oxygen species (e.g., 2-methoxyestradiol, which increases intracellular superoxide anion). Other Notes Depletes glutathionine in isolated cells