Description_x000D_ General description_x000D_ Duolink™ In Situ Microplate Heat Transfer Block is an aluminium block ideal for keeping an even temperature across the plate during the incubation steps when using Duolink In Situ reagents in multiwell plates. Incubation in the pre-heated Heat Transfer Block increases staining efficiency and reproducibility between different plates, and reduces plate edge effects._x000D_ Application_x000D_ Duolink™proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples._x000D_ This product can be applied to both the Duolink™ In Situ Fluorescence Protocol and the Duolink™ In Situ Brightfield Protocol depending on the detection reagents used._x000D_ Visit our Duolink™ PLA Resource Center for information on how to run a Duolink™ experiment, applications, troubleshooting, and more._x000D_ To perform a complete Duolink™ PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink™ PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection._x000D_ Specificity_x000D_ Duolink™ In Situ Microplate Heat Transfer Block will increase staining efficiency and reproducibility between different plates and reduce edge effects when using Duolink™ reagents in microtiter plates._x000D_ Keep the Heat Transfer Block preheated at 37°C and keep it in the 37°C incubator throughout the whole Duolink™ In Situ protocol. Place the microtiter plate in the Heat Transfer Block during incubation steps._x000D_ Application Note_x000D_ Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink™ in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required._x000D_ Linkage_x000D_ Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink™ projects_x000D_ View full Duolink™ product list_x000D_ Features and Benefits_x000D_ • No overexpression or genetic manipulation required_x000D_ • High specificity (fewer false positives)_x000D_ • Single molecule sensitivity due to rolling circle amplification_x000D_ • Relative quantification possible_x000D_ • No special equipment needed_x000D_ • Quicker and simpler than FRET_x000D_ • Increased accuracy compared to co-IP_x000D_ • Publication-ready results_x000D_ Preparation Note_x000D_ Storage and Stability: When not in use, store the Heat Transfer Block at room temperature._x000D_ Legal Information_x000D_ Duolink is a trademark of Sigma-Aldrich Co. LLC_x000D_ PLA is a registered trademark of Sigma-Aldrich Co. LLC