Description Specificity Recognizes Endothelin Receptor B (ET-B). The immunogen sequence is specific for ET-B and is not present in any other known protein. SPECIES REACTIVITIES: The immunogen sequence is identical in all known vertebrate proteins. Reactivity with other species has not been confirmed. Immunogen Purified peptide corresponding to amino acids 298-314 of the rat Endothelin Receptor B (Accession P21451). Application Anti-Endothelin Receptor B Antibody detects level of Endothelin Receptor B & has been published & validated for use in IH & WB. Research Category Neuroscience Research Sub Category CNS Control of Metabolism Hormones & Receptors Western blot: 1:200 using ECL on rat brain membranes. Immunohistochemistry on rat brain sections. Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. Physical form Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). Storage and Stability Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Disclaimer Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Analysis Note Control Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. Other Notes Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.