Description_x000D_ Application_x000D_ Enhanced Avian Reverse Transcriptase (eAMV RT) is used to transcribe RNA into DNA, and facilitates efficient mRNA template driven sysnthesis of cDNAs. This is due to the abillity of this enhanced AMV-RT to transcribe large mRNA templates, to transcribe through difficult secondary structures, and to detect low abundance mRNAs by RT-PCR._x000D_ Biochem/physiol Actions_x000D_ Reverse transcriptase catalyzes RNA template incorporation of dNTPs into complimantary DNA through phosphodiester bond formation._x000D_ Features and Benefits_x000D_ • Greater sensitivity for low abundance mRNA_x000D_ • Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)_x000D_ • Efficient generation of full-length cDNA, up to 14.1 kb_x000D_ Packaging_x000D_ Provided with a vial of 10× reaction buffer._x000D_ Unit Definition_x000D_ One unit incorporates one nanomole of TMP into TCA precipitable material in 10 min using polyadenylic acid as template and oligo(dT)12-18 as a primer._x000D_ Legal Information_x000D_ Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler._x000D_ eAMV is a trademark of Sigma-Aldrich Co. LLC