Description_x000D_ General description_x000D_ EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody covalently attached to crosslinked agarose beads._x000D_ Specificity_x000D_ Binding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C). It recognizes reduced forms of HA-tagged proteins and is reactive with N or C-terminal HA-tagged fusion proteins._x000D_ Application_x000D_ EZview™ Red Anti-HA Affinity Gel has been used for immunoblot analysis and immunoprecipitation assay._x000D_ When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost._x000D_ Physical form_x000D_ 1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon_x000D_ Legal Information_x000D_ EZview is a trademark of Sigma-Aldrich Co. LLC_x000D_ Features and Benefits_x000D_ • Increased visibility - Red color reduces risk of incidental aspiration_x000D_ • Improved recovery of target protein by reduced accidental loss_x000D_ • Higher reproducibility - More consistent yields_x000D_ Other Notes_x000D_ The affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA).