Description General description Recombinant, human Fas ligand (amino acids 103-281) fused to a 26-amino acid linker and FLAG®-tag at the N-terminus and expressed in HEK293 cells. Does not require enhancer for activity. Suitable for cytotoxicity assays. M.W. 32,000 - 35,000. Recombinant, human Fas ligand (amino acids 103-281) fused to a 26-amino acid linker and FLAG®-tag at the N-terminus and expressed in HEK293 cells. Glycosylation of recombinant human sFasL is similar or identical to natural human FasL. The molecular weight is ~32 kDa (nonglycosylated) and ~37-42 kDa (glycosylated) by SDS-PAGE under reducing conditions. The recombinant protein is produced in HEK293 cells. It does not require enhancer for activity. Useful for cytotoxicity assays. Fas Ligand (FasL/APO-1L/CD95L) is a ~37-42 kDa type II transmembrane protein belonging to the TNF family. Interaction between FasL and Fas induces apoptosis of Fas-sensitive cells. FasL is implicated in CTL-mediated-killing, activation-induced cell death, creation of immune-privileged sites and tissue homeostasis. The extracellular domain of human FasL can be cleaved off by a metalloprotease, generating soluble FasL (sFasL). Circulating sFasL can be detected in serum of patients with melanoma and certain types of lymphoma. Packaging 5 μg in Plastic ampoule Biochem/physiol Actions ≥1 ng/ml kills Fas-sensitive cells Warning Toxicity: Standard Handling (A) Physical form Lyophilized from PBS. Reconstitution Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Reconstitute in 50 µl PBS to yield a final stock concentration of 100 µg/ml. Further dilute in culture medium with 5% FCS immediately prior to use. Other Notes Ashkenazi, A., and Dixit, V.M. 1998. Science281, 1305. Nagata, S., 1997 Cell88, 355. Hahne, M., et al. 1996. Science274, 1363. Bellgrau, D., et al. 1995. Nature377, 630. Lowin, B., et al. 1994. Nature370, 650. Russell, J.H., et al. 1993. Proc. Natl. Acad. Sci. USA90, 4409. Glycosylation of recombinant human sFasL is similar or identical to natural human FasL. The molecular weight is ~32 kDa (nonglycosylated) and ~35 kDa (glycosylated) by SDS-PAGE under reducing conditions. The recombinant protein is produced in HEK293 cells. It does not require enhancer for activity. Legal Information FLAG is a registered trademark of Sigma-Aldrich Co. LLC