Description_x000D_ General description_x000D_ Extinction Coefficient: E1% = 21.0 (280 nm)_x000D_ pI: 9.0_x000D_ Application_x000D_ Ficin from fig tree latex has been used:_x000D_ • in the proteolysis of tendon collagen_x000D_ • in the digestion of Chlorophyta Ulva rigida_x000D_ • to investigate it effect on protease-activated receptors 2 and 4 activation to induce itch in skin_x000D_ Packaging_x000D_ 100, 200, 1000 units in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab')2 fragments from mouse IgG1._x000D_ Ficin is effective in disrupting the staphylococcal biofilms making it accessible antibiotics exposure._x000D_ Unit Definition_x000D_ One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path)._x000D_ Physical form_x000D_ Lyophilized powder containing L-cysteine and trace EDTA_x000D_ Preparation Note_x000D_ This enzyme is soluble in water (10 mg/ml), yielding a clear solution.