Description_x000D_ General description_x000D_ Ficoll 400 is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400 is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400 is very hydrophilic and extremely water-soluble. The most common application for Ficoll 400 is as a density gradient medium for the separation and isolation of eukaryotic cells, organelles and bacterial cells. Density ranges up to 1.2 g/ml can be attained. It has also been utilized in a variety of other applications._x000D_ Application_x000D_ Ficoll® PM 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque a density mixture used for the separation of cells and organelles, especially blood derived lymphocytes and monocytes. It may be used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations. Ficoll PM-400 may be used in cell freeze-drying research._x000D_ Used for cell separation and organelle isolation._x000D_ Packaging_x000D_ 10, 25, 100, 500 g in poly bottle_x000D_ Features and Benefits_x000D_ Because of the high molecular weight and low content of dialyzable material, Ficoll has a much lower permeability towards cell membranes than sucrose. Therefore, cells can be expected to collect at a lower density in Ficoll gradients than in sucrose gradients. Because of its low membrane permeability and low osmotic pressure, separations in Ficoll normally result in better preservation of cell function and morphology._x000D_ Other Notes_x000D_ A nonionic synthetic polymer of sucrose._x000D_ Preparation Note_x000D_ Concentrations of 50% (w/v) can be attained in water. Ficoll should be added slowly with constant stirring._x000D_ Sigma tests the solubility of Ficoll 400 at 1 g in 10 ml of deionized water yielding a clear to slightly hazy, colorless to faint yellow solution. Ficoll is stable in alkaline and neutral solutions. At pH values below 3, it is rapidly hydrolyzed, particularly at elevated temperatures. Ficoll can be sterilized by autoclaving at a neutral pH, at 110oC for 30 minutes. Strong oxidizing and reducing agents are to be avoided._x000D_ Legal Information_x000D_ Ficoll is a registered trademark of Cytiva