Description_x000D_ General description_x000D_ Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type._x000D_ Fpg contains two domains separated by a flexible hinge._x000D_ Application_x000D_ Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay._x000D_ Packaging_x000D_ 10 μg in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase._x000D_ Fpg specifically acts on 3′- and 5′-phosphodiester bonds._x000D_ Unit Definition_x000D_ One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine−mutated) in 10 min at 25 °C._x000D_ Physical form_x000D_ Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl.