Description_x000D_ General description_x000D_ Chemical structure: aminoglycoside_x000D_ Application_x000D_ G418 disulfate is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL._x000D_ Biochem/physiol Actions_x000D_ Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes._x000D_ Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes._x000D_ Caution_x000D_ The product is stable for two years when stored at 2-8°C.