Description_x000D_ General description_x000D_ Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region._x000D_ Application_x000D_ Galactosyltransferase from bovine milk has been used:_x000D_ • in the generation of hyper-galactosylated variant of IgG1_x000D_ • in β -1,4-galactosyltransferase assay rice root cell extracts_x000D_ • in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers_x000D_ Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine._x000D_ Packaging_x000D_ 25 units in glass bottle_x000D_ 5, 10 units in serum bottle_x000D_ Biochem/physiol Actions_x000D_ Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides._x000D_ Galactosyltransferase from bovine milk requires Mn2+ ions for its catalytic functionality. Alteration in the galactosyltransferase levels have been implicated in various diseases states._x000D_ Linkage_x000D_ This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90._x000D_ Unit Definition_x000D_ One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)_x000D_ Physical form_x000D_ Lyophilized powder containing Tris, EDTA, and (NH4)2SO4