Description_x000D_ General description_x000D_ Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants._x000D_ Application_x000D_ Gamborg’ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg’s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg’s B-5 medium has been used for the culture of Annona squamosa callus._x000D_ Gamborg′s B-5 basal medium with minimal organics has been used in culturing plant cells of Daucus carota. It has also been used for selection of primary transformants of Arabidopsis thaliana._x000D_ Formula variant_x000D_ With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968)._x000D_ Media Formulation_x000D_ Quantity_x000D_ Formulated to contain 3.2 grams of powder per liter of medium.