Description_x000D_ Amino Acid Sequence_x000D_ Cer-Glc-Gal(NeuAc-NeuAc)-GalNAc-Gal-NeuAc_x000D_ General description_x000D_ Gangliosides are major constituents of neuronal cell membranes and endoplasmic reticulum. They contain a sialated polysaccharide chain linked to ceramide through a β-glycosidic linkage. For classification of gangliosides see Svennerholm, L., et al. (eds.), Structure and Function of Gangliosides, New York, Plenum, 1980._x000D_ Application_x000D_ Trisialoganglioside-GT1b from bovine brain has been used:_x000D_ • in high performance thin layer chromatography (HPTLC)_x000D_ • in membrane GM1 assays_x000D_ • as reference standards for TLC (thin layer chromatography) and as antigens for ELISA (enzyme-linked immunosorbent assay)_x000D_ Packaging_x000D_ 1, 5 mg in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Ganglioside marker for metastatic brain tumors. Modulates cellular differentiation; prevents glutamate neurotoxicity; inhibits mitogenesis stimulated by lectins such as Con A. Converted to GD1b by bacterial and mammalian sialidases._x000D_ Analysis Note_x000D_ Homogeneous aqueous preparations can be obtained by suspending the compound in water, buffer or cell culture medium and sonicating briefly to facilitate the micelle formation.