Description_x000D_ General description_x000D_ Gc-Globulin, a member of the albumin superfamily, is the major plasma carrier of vitamin D and its metabolites. It is also, along with gelsolin, part of the extracellular actin scavenger system. Gc-globulin also binds monomeric actin (G-actin) with high affinity to scavenge actin released by necrotic cells, preventing its polymerization into filaments. Gc-globulin enhances the chemoattractant activity of C5a for inflammatory cells._x000D_ Gc-globulin from human plasma is encoded by the gene GC, which is mapped to human chromosome 4q13.3._x000D_ Gc-globulin or the vitamin D binding protein (DBP) is a 51-58 kDa multifunctional serum glycoprotein synthesized in large quantities by primarily in the liver. It is a member of the albumin gene family and has the characteristic multiple disulfide-bonded, triple domain structure._x000D_ Application_x000D_ Gc-globulin from human plasma has been used:_x000D_ • as a fluorescent probe for G-actin staining_x000D_ • as a fluorescent probe to monitor steady-state rate of actin filament disassembly_x000D_ • as a calibrator for liquid chromatography with mass spectrometry (LC-MS/MS)_x000D_ Gc-globulin is a multifunctional, albumin-like plasma protein that often requires cell surface binding to mediate some of its diverse functions. It has been used to anlayze its glycosylation patterns._x000D_ Biochem/physiol Actions_x000D_ Gc-Globulin, a member of the albumin superfamily, is the major plasma carrier of vitamin D and its metabolites. It is also, along with gelsolin, a part of the extracellular actin scavenger system. Gc-globulin also binds monomeric actin (G-actin) with high affinity to scavenge actin released by necrotic cells, preventing its polymerization into filaments. Gc-globulin enhances the chemoattractant activity of C5a for inflammatory cells._x000D_ Single nucleotide polymorphism in the gene GC is associated with deficiency of 25-hydroxyvitamin D [25(OH)D]._x000D_ Preparation Note_x000D_ Native Gc-globulin from human plasma