Description_x000D_ General description_x000D_ The GenElute Blood Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform._x000D_ Application_x000D_ GenElute™ Blood Genomic DNA Kit has been used:_x000D_ • to extract DNA from samples of large granules (LG) and smaller granules (SG) obtained from vasectomized rabbits_x000D_ • to isolate DNA from the blood samples_x000D_ • to extract whole blood DNA from the left blood cells_x000D_ The purified genomic DNA is ready for downstream applications such as:_x000D_ • restriction endonuclease digestions_x000D_ • PCR_x000D_ • Southern blots_x000D_ • sequencing reactions_x000D_ • cloning_x000D_ Features and Benefits_x000D_ • Starting material: Up to 200 μl of fresh or aged blood_x000D_ • Expected yield: Up to 10 μg_x000D_ • Elution volume: 400 μl_x000D_ • Time required: <40 min_x000D_ • A260/A280 ratio: 1.6 - 1.9_x000D_ • Compatible with many anticoagulants, including EDTA, Heparin, and Sodium Citrate_x000D_ Principle_x000D_ The starting material is lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. A Prewash Solution is provided to help remove contaminants that are associated with aged (older than 24 hours) whole blood samples. After washing to remove contaminants, the DNA is eluted in 200 mL of a Tris-EDTA solution._x000D_ The expected yields of genomic DNA will vary depending on the amount and nature of the starting material used (for example, 4 to 10 μg of RNase A-treated DNA can be isolated from 200 μl of fresh whole blood in less than one hour). DNA purified with this kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length._x000D_ Other Notes_x000D_ For additional information, please see www.sigma-aldrich.com/genomicdna._x000D_ Legal Information_x000D_ GenElute is a trademark of Sigma-Aldrich Co. LLC