Description_x000D_ General description_x000D_ The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format._x000D_ Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform._x000D_ Application_x000D_ The purified genomic DNA is ready for immediate use in downstream applications such as:_x000D_ • restriction endonuclease digestions_x000D_ • PCR_x000D_ • Southern blots_x000D_ • sequencing reactions_x000D_ • cloning_x000D_ • ligation_x000D_ Features and Benefits_x000D_ • Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue_x000D_ • Elution volume: 200 - 400 μl_x000D_ • Time required: 20 min after lysis_x000D_ • A260/A280 ratio: 1.6 - 1.9_x000D_ • Mechanical homogenization required: No_x000D_ Principle_x000D_ The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length._x000D_ Other Notes_x000D_ For additional information, please see www.sigma-aldrich.com/genomicdna._x000D_ Legal Information_x000D_ GenElute is a trademark of Sigma-Aldrich Co. LLC