Description_x000D_ General description_x000D_ Sigma’s GenElute™ Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays._x000D_ • Isolate true total RNA including siRNA and microRNA_x000D_ • No phenol extractions_x000D_ • Purify high-quality RNA in 40 minutes_x000D_ • Isolate total RNA from a wide variety of sources_x000D_ Application_x000D_ • Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA_x000D_ • RT-PCR for both large mRNA and small RNA including miRNA_x000D_ • Expression profiling_x000D_ • Next Generation Sequencing for RNA and miRNA_x000D_ • miRNA from plasma for discovery_x000D_ • microRNA cloning and amplification_x000D_ • PCR-based virus detection_x000D_ • PCR-based viable bacteria detection_x000D_ • Northern blotting_x000D_ • RNase protection_x000D_ • Primer extension_x000D_ • miRNA array_x000D_ • RNA-Seq_x000D_ • Nanostring_x000D_ • Fluidigm_x000D_ • Droplet/Digital PCR_x000D_ Features and Benefits_x000D_ • Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)_x000D_ • Fast and easy processing - Rapid spin-column format allows for the processing of 4 samples in under 40 minutes._x000D_ • No phenol:chloroform extractions – Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform._x000D_ • Isolate total RNA from a wide variety of sources – Total RNA can be isolated from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses using this kit._x000D_ • Isolate inhibitor-free RNA suitable for downstream applications - Purified RNA can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array analysis requiring the use of intact RNA_x000D_ • Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)_x000D_ • Fast and easy processing - Rapid spin-column format allows for the processing of 4 samples in under 40 minutes._x000D_ • No phenol:chloroform extractions – Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform._x000D_ • Isolate total RNA from a wide variety of sources – Total RNA can be isolated from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses using this kit._x000D_ • Isolate inhibitor-free RNA suitable for downstream applications - Purified RNA can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array analysis requiring the use of intact RNA_x000D_ Unit Definition_x000D_ Preps_x000D_ Other Notes_x000D_ We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions._x000D_ Legal Information_x000D_ GenElute is a trademark of Sigma-Aldrich Co. LLC