Description_x000D_ General description_x000D_ The kit provides all reagents for simple and quick assay to measure the level of total glutathione (GSSG + GSH) in a cell and tissue extracts and in red blood cells or plasma. The sample is first deproteinized with the 5% 5-sulfosalicylic acid solution. Glutathione content of the sample is then assayed using a kinetic assay in which catalytic amounts of glutathione cause a continuous reduction of 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB) to TNB. The oxidised glutathione formed is recycled by glutathione reductase and NADPH. The product, TNB, is assayed colorimetrically at 412 nm._x000D_ Application_x000D_ Glutathione Assay Kit has been used to measure total glutathione concentration in glutathione assay. It also has been used to determine different oxidative stress parameters in rats._x000D_ Biochem/physiol Actions_x000D_ Glutathione (GSH) functions as a cofactor for several antioxidant enzymes. It is involved in the regeneration of vitamins C and E. It modulates cellular proliferation and apoptosis. GSH is crucial for mitochondrial functioning and the maintenance of mitochondrial DNA (mtDNA). Mutations in this gene are associated with Alzheimer′s, Parkinson′s and Huntington′s diseases. It is also linked to cystic fibrosis, immune diseases and cardiovascular diseases._x000D_ Reduced glutathione (GSH), a tripeptide (g-glutamyl-cysteinylglycine), is the major free thiol in most living cells and is involved in many biological processes such as detoxification of xenobiotics, removal of hydroperoxides, and maintenance of the oxidation state of protein sulfhydryls. It is the key antioxidant in animal tissues_x000D_ Features and Benefits_x000D_ • A quick and simple method_x000D_ • The kit contains all the reagents required for a colorimetric assay of total glutathione (oxidised + reduced GSSG + GSH)_x000D_ • The kit includes a deproteination reagent for biological samples