Description_x000D_ General description_x000D_ Glutathione S-transferase (GST) is a major detoxification enzyme, and exists as multiple cytoplasmic and membrane-bound isozymes. Cytoplasmic isoforms of GST are encoded by five genes, namely α, γ, μ, σ and π. α, μ and π are the most abundant forms in mammals. GSTπ is the isoform of this enzyme found in placenta and is composed of 209 amino acids._x000D_ Glutathione S-transferase mu 1 (GSTM1) is mapped to human chromosome 1p13.3._x000D_ Application_x000D_ Glutathione S-Transferase from human placenta has been used for the immunization of rabbits for the production of specific anti-π-GST antibodies, to determine whether total anti-π-GST antibodies are more specific and reactive as compared to anti-peptide antibodies._x000D_ Glutathione S-Transferase from human placenta has been used in antigen microarray and informatics analysis._x000D_ Packaging_x000D_ 1, 5, 10 mg in glass bottle_x000D_ Biochem/physiol Actions_x000D_ Glutathione S-transferase (GST) catalyzes conjugation reactions during phase II metabolism. This enzyme also confers protection against catabolic products of peroxidized lipid and oxidized DNA, and ROS (reactive oxygen species) or electrophiles._x000D_ Glutathione S-transferase (GST) catalyzes the reduction of dehydroascorbic acid to ascorbic acid in the presence of glutathione. GST exhibits neuroprotective action, by eliminating oxidants (exogenous and endogenous), detoxifying therapeutic drugs and other toxins in the environment. Mutation in this gene is known to cause epilepsy. Deletion of the gene may result in cancer._x000D_ Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers._x000D_ Unit Definition_x000D_ One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C._x000D_ Physical form_x000D_ Lyophilized powder containing Tris buffer salts, reduced glutathione and EDTA._x000D_ Analysis Note_x000D_ Protein determined by biuret._x000D_ Purified and assayed by a modification of the method of Simons and Vander Jagt._x000D_ Enzymatic activities are based on the conjugation of reduced glutathione with a second substrate. The individual proteins generally have activity with more than one class of substrate.