Description_x000D_ Packaging_x000D_ 1000 units in poly bottle_x000D_ Application_x000D_ This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase (=G-3-P DH, G3D-301), glycerol-3-phosphate oxidase (=G-3-P oxidase, G3O-301, G3O-311, G3O-321) or pyruvate kinase (PYK-301) and lactate dehydrogenase (LCD-209, LCD-211), lipoprotein lipase (LPL-311, LPL-314) in clinical analysis._x000D_ Biochem/physiol Actions_x000D_ Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate._x000D_ Physical properties_x000D_ Isoelectric point : 4.2_x000D_ Michaelis constants : 4.4 x 10-5M (Glycerol), 4.3 x 10-4M (ATP)_x000D_ Inhibitors : p-Chloromercuribenzoate, heavy metal ions (Pb++, Fe++, Hg++, Ag+)_x000D_ Optimum pH : 9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system) Optimum temperature : 500C_x000D_ pH Stability : pH 5.5 x 10.0 (25oC, 20hr)_x000D_ Thermal stability : below 40oC (pH 7.5, 15min)_x000D_ Substrate specificity : This enzyme catalyzes the stereospecific transfer of the terminal_x000D_ phosphoryl moiety of ATP to one of the primary hydroxyl group of_x000D_ glycerol, forming sn-glycerol-3-P. The enzyme has the highest_x000D_ specificity for glycerol, and also phosphorylates dihydroxyacetone_x000D_ and glyceraldehyde (Table 1,2). Mg++ is essentially required for the_x000D_ reaction._x000D_ Unit Definition_x000D_ One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH._x000D_ Physical form_x000D_ Lyophilized powder containing phosphate buffer salts and sodium gluconate