Description General description Avanti Lipid Snoopers® are lipid microarrays with lipid spotted on solid support and are useful in semi-quantitative measurements. These snoopers are relatively stable and provide high sensitivity compared to the conventional enzyme-linked immunosorbent assay (ELISA) methods. Ganglioside GM1 (monosialotetrahexosylganglioside) is present in the brain and comprises one sialic acid moiety and unsaturated acyl chains. GM1 is catabolized by the enzyme GM1 β-galactosidase. ELISA Snoopers® are 8-well strips pre-coated with a single high-quality lipid species. They can be used to investigate protein-lipid interactions, and characterize and titrate lipid binding proteins. This format provides the following advantages: • quantitative lipid-protein binding studies • assemble a 96-well plate using only the lipids of interest for binding studies • flexibility - use 8 to 96 wells, and 1 to 12 lipids as needed. The 8-well strips are available in optically clear, white, or black surfaces for use in colormetric, luminescent and fluorescent assays, respectively. Avanti Lipid Snoopers ELISA Strips allow multiple sample conditions to be probed simultaneously in a single plate making them a perfect complement to Avanti Lipid Snoopers Nitrocellulose Strips. Biochem/physiol Actions Avanti Lipid Snoopers® aid in probing protein–lipid interaction by chemiluminescent detection method. Snoopers® strips are useful in characterizing lipid-binding antibodies and decoding cell signalling pathways. Ganglioside GM1 prevents the formation of aggregating cytotoxic fibrils from α-synuclein in Parkinson′s disease. The deficiency of the GM1 β-galactosidase results in the accumulation of GM1 in lysosomes leading to GM1 gangliosidosis. High levels of GM1 leads to neuronal degeneration. GM1 supplementation improves cognitive function in Alzheimer′s disease patients. Packaging ELISA Strip Packaged in Foil Pouch (330651-1EA) Legal Information Snoopers is a registered trademark of Avanti Polar Lipids, Inc.