Description_x000D_ General description_x000D_ The antibody recognizes cell surface mouse TNF RI (55 kDa) and exhibits TNF agonist activities. The antibody shows < 5% cross-reactivity with recombinant human TNF sRI and no cross-reactivity with recombinant human TNFsRII._x000D_ Immunogen_x000D_ recombinant mouse TNF soluble receptor I, expressed in Escherichia coli._x000D_ Physical form_x000D_ Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose._x000D_ Biochem/physiol Actions_x000D_ The biological effects of tumor necrosis factor soluble factor (TNSF) are mediated by either type I or type II receptors. Type I receptors that are ubiquitously present on all cells whereas type II receptors are expressed only on cells of immune system and endothelial cells. Receptors of TNFSF such as TNFR1 and Fas have their own death domains that activate the apoptosis. Other receptors such as TNFR2 do not have death domains and utilize TNF associated factors (TRAFs) to activate intracellular signaling. The pathways activated via TNFsRI include NF-κB, JNK and MAPK.