Description_x000D_ Application_x000D_ Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase._x000D_ Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary._x000D_ Packaging_x000D_ 1 kg in poly bottle_x000D_ 2, 10, 25 kg in poly drum_x000D_ 25, 100, 500 g in poly bottle_x000D_ Caution_x000D_ May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical. For free-flowing material, please see our Redi-Dri™ free-flowing product number RDD001._x000D_ Preparation Note_x000D_ In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes._x000D_ The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M._x000D_ Legal Information_x000D_ Redi-Dri is a trademark of Sigma-Aldrich Co. LLC