Description_x000D_ Application_x000D_ Guanidine thiocyanate has been used:_x000D_ • for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation_x000D_ • as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay_x000D_ • for DNA extraction from cerebrospinal fluid (CSF)_x000D_ Chaotropic agent and strong denaturant; solubilizes cells._x000D_ Packaging_x000D_ 100, 250, 500 g in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.