Description_x000D_ General description_x000D_ Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation._x000D_ Application_x000D_ Hanahan′s Broth (SOB Medium) has been used for the preparation of C. necator H16 competent cells._x000D_ Biochem/physiol Actions_x000D_ Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli._x000D_ Features and Benefits_x000D_ Hannahan’s Broth (SOB) powder provides:_x000D_ • Standard formulation_x000D_ • An inexpensive alternative to liquid_x000D_ Components_x000D_ 20 g/L Tryptone_x000D_ 5 g/L Yeast Extract_x000D_ 2.4 g/L MgSO4_x000D_ 0.5g/L NaCl_x000D_ 0.186 g/L KCl_x000D_ Reconstitution_x000D_ Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).