Description_x000D_ General description_x000D_ HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG MRP2 knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways._x000D_ Application_x000D_ See technical bulletin for detailed protocols_x000D_ Features and Benefits_x000D_ HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity. - Sigma′s HepaRG MRP2 Knockout (KO) allows investigations of drug-transporter interactions involving MRP2 in the liver._x000D_ Zinc finger nucleases (ZFN) mediated Knockout of ABCC2 (MRP2) gene._x000D_ • The frame-shift mutation of ABCC2 gene was confirmed by fragment length analysis and DNA sequencing._x000D_ • Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay._x000D_ Quality_x000D_ Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation._x000D_ Legal Information_x000D_ Exhibit 1: ADME/Tox cell lines license_x000D_ Exhibit 2: HepaRG limited use license_x000D_ HepaRG is a trademark of BioPredic International company