Description_x000D_ General description_x000D_ Heparinase is an inducible, non-extracellular heparin-degrading enzyme. Three types of heparinises are produced by Flavobacterium heparinum and contains specific sequences of heparin._x000D_ Application_x000D_ Heparinase I and III Blend from Flavobacterium heparinum has been used in:_x000D_ • the digestion of heparan sulfate from ovine vitreous_x000D_ • human embryonic kidney cells_x000D_ • glycosaminoglycans from arterial tissues_x000D_ • P0 retinae digestion_x000D_ Biochem/physiol Actions_x000D_ Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization. It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides._x000D_ Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate._x000D_ Packaging_x000D_ Sold on the basis of Heparinase I units_x000D_ Unit Definition_x000D_ One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using Heparin, Sodium as substrate for heparinase I._x000D_ One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using bovine kidney Heparan, Sulfate as substrate for heparinase III._x000D_ One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units._x000D_ Other Notes_x000D_ Enzyme Commission Numbers: 4.2.2.7 Hep I and 4.2.2.8 Hep III