Description_x000D_ Application_x000D_ Heparinase I from Flavobacterium heparinum has been used:_x000D_ • in the digestion of plasma samples_x000D_ • to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumonia_x000D_ • in enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells_x000D_ Biochem/physiol Actions_x000D_ Heparinase I from Flavobacterium heparinum acts by cleaving the α-glycoside linkage at AT III binding site in heparin. This results in small oligosaccharide fragments and heparinase I neutralizes the protein′s action of anticoagulation. Therefore it might replace the use of protamine in reversing heparin induced anticoagulation after coronary artery bypass surgery. Heparinase I possesses a half-life of 15-18 minutes._x000D_ Unit Definition_x000D_ One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units._x000D_ Other Notes_x000D_ View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer