Description_x000D_ General description_x000D_ HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg._x000D_ Application_x000D_ HEPES has been used:_x000D_ • In the novel vesicle-budding protocol as a component in stock solution and buffer solutions_x000D_ • As a component in intracellular solution for the preparation of brain-slice recording_x000D_ • As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification_x000D_ • To maintain the pH at 7 in thermal denaturation assay of purified protein_x000D_ • In isocratic elution of protein using gel filtration_x000D_ • Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy_x000D_ Packaging_x000D_ 1 kg in poly bottle_x000D_ 2.5 kg in poly drum_x000D_ Legal Information_x000D_ Redi-Dri is a trademark of Sigma-Aldrich Co. LLC