Description_x000D_ General description_x000D_ HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures._x000D_ HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg._x000D_ Application_x000D_ HEPES has been used:_x000D_ • To supplement Dulbecco’s modified Eagle’s medium to culture and maintain cell lines_x000D_ • As a component of platelet suspension buffer_x000D_ • To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue_x000D_ • As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay_x000D_ • For the adjustment and maintenance of pH of biological solutions_x000D_ • As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development_x000D_ • For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells_x000D_ • As a component of keratinocyte and fibroblast culture medium_x000D_ Packaging_x000D_ 1 kg in poly bottle_x000D_ 100, 500 g in poly bottle_x000D_ 25 kg in poly drum_x000D_ Other Notes_x000D_ Easily compare specifications for HEPES products with the HEPES specification table.