Description General description HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. Application HEPES has been used: • As a component of SAPI medium used for the culturing of S. Typhymurium inoculum • To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI • As a component of dissection buffer for culturing rat cortical neurons • Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses • As a component of Danieu′s aquarium water used in agarose gel preparation