Description_x000D_ General description_x000D_ HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg._x000D_ Application_x000D_ HEPES has been used:_x000D_ • As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo_x000D_ • As a component of Hanks’ solution used in the degradation testing of Iron_x000D_ • As a component of HEPES medium in the transfection of cell culture with plasmids_x000D_ • Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells_x000D_ • As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies_x000D_ Packaging_x000D_ 1 kg in poly bottle_x000D_ 25, 100, 250, 500 g in poly bottle_x000D_ 5, 25, 50 kg in poly drum_x000D_ Other Notes_x000D_ Go to Redi-Dri™ Product Listing RDD002_x000D_ Legal Information_x000D_ Redi-Dri is a trademark of Sigma-Aldrich Co. LLC