Description_x000D_ General description_x000D_ PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit sigma-aldrich.com/safc-pharmagrade._x000D_ Packaging_x000D_ 100 g in poly bottle_x000D_ 5, 10, 25, 50 kg in poly drum_x000D_ Application_x000D_ HEPES is a biological buffer often referred to as a “Good’s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high solution clarity._x000D_ HEPES is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors._x000D_ Linkage_x000D_ Enhanced Quality Product Documentation on PharmaGrade HEPES