Description_x000D_ General description_x000D_ The HIS-Select Cobalt Affinity Gel is a quadridentate chelate on beaded agarose that is charged with cobalt. The gel is designed to specifically bind histidine containing proteins. It is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins._x000D_ HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with histidine tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/ml of packed gel, determined with a histidine-tagged protein, molecular mass ~30 kDa._x000D_ Application_x000D_ HIS-Select Cobalt Affinity Gel allows for high purity, low non-specific binding, and high binding capacity of protein. HIS-Select Cobalt Affinity Gel also works well for purification of histidine-tagged proteins in native, denaturing, or mild reducing conditions. HIS-Select™ Cobalt Affinity Gel has been used to purify His-tagged proteins._x000D_ Packaging_x000D_ 5, 100 mL in poly bottle_x000D_ Physical form_x000D_ 1:1 suspension in a 30% ethanol solution_x000D_ Legal Information_x000D_ HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC_x000D_ HiScreen is a trademark of Cytiva