Description_x000D_ General description_x000D_ Sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) are provided as purified plasmid DNA suitable for virus production and transient transfection. DNA is provided in 40 μl aliquots per well in Tris,EDTA (TE) solution. An average of 2 μg per clone is provided per well with a range from 400 ng to 4 μg. The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set._x000D_ Other Notes_x000D_ Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene._x000D_ For a detailed listing of other available gene family sets, visit the gene family set website._x000D_ Number of Genes: 368, Number of Clones: 2396_x000D_ The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated._x000D_ Legal Information_x000D_ Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense ._x000D_ MISSION is a registered trademark of Sigma-Aldrich Co. LLC