Description_x000D_ General description_x000D_ Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery._x000D_ Application_x000D_ Hyaluronidase from sheep testes has been used:_x000D_ • in flow cytometry_x000D_ • in the isolation of putative spermatogonial stem cells (SSCs)_x000D_ • to study the effects of magnetic stimulation in magnetic hydrogel properties_x000D_ • in immunohistochemistry to visualize collagen type II distribution_x000D_ • to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings_x000D_ • for the degeneration of hydrogels_x000D_ Packaging_x000D_ 1, 5 g in poly bottle_x000D_ 100, 500 mg in poly bottle_x000D_ Biochem/physiol Actions_x000D_ These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates._x000D_ Unit Definition_x000D_ One unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay)._x000D_ Physical form_x000D_ Lyophilized powder containing lactose_x000D_ Other Notes_x000D_ View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer