Description_x000D_ General description_x000D_ Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region._x000D_ Application_x000D_ Hyaluronidase has been used:_x000D_ • as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules_x000D_ • in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry_x000D_ • in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines_x000D_ Packaging_x000D_ 1 g in poly bottle_x000D_ 500 mg in poly bottle_x000D_ Biochem/physiol Actions_x000D_ Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively._x000D_ These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates._x000D_ Unit Definition_x000D_ One unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay)._x000D_ Other Notes_x000D_ View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer