Description_x000D_ General description_x000D_ Chemical structure: aminoglycoside_x000D_ Application_x000D_ Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes._x000D_ Biochem/physiol Actions_x000D_ Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation._x000D_ Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells._x000D_ Caution_x000D_ Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days._x000D_ Preparation Note_x000D_ This product is a solution that is =60% (HPAE), 45-60 mg/mL in H2O, and γ-irradiated .